All WGS libraries for E. flavifrons sample Harlow, the source of the ... - SRA

SRX1037935: All WGS libraries for E. flavifrons sample Harlow, the source of the reference genome
1 ILLUMINA (Illumina HiSeq 2000) run: 797.3M spots, 114G bases, 60.3Gb downloads

Design: PE libraries of target insert sizes 180 bp, 500 bp, and 1 kb were prepared using the recommended Illumina protocols at the University of Chicago and sequenced using an Illumina HiSeq2000 at Princeton University (Princeton, NJ). PE libraries with mean target insert size of 1 kb were prepared with Illumina’s TruSeq DNaseq Sample prep kit and MP libraries were prepared with Illumina’s Mate-pair version 2 Library prep kit at the Keck Sequencing Center of the University of Illinois (Champaign, IL); these libraries were also sequenced at the Keck Center. PE libraries were quantitated by qPCR and sequenced for 100 cycles from each end of the fragments on a HiSeq2000 machine using a TruSeq SBS sequencing kit version 3. Reads were analyzed with Casava 1.8 (pipeline 1.9) and subsequently stripped of adaptors.

Submitted by: University of Chicago

Study: Whole genome sequence of Eulemur flavifrons and Eulemur macaco

PRJNA284191 • SRP058683 • All experiments • All runs

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Our lab has used next-generation sequencing technology to produce high coverage genome-wide sequence data for a single Eulemur flavifrons individual and a single Eulemur macaco individual, in order to address questions related to the demographic history of the two species, as well as to identify candidate regions that may influence their divergent pigmentation phenotypes.

Sample:

SAMN03699688 • SRS945120 • All experiments • All runs

Organism: Eulemur flavifrons

Library:

Name: EfHarlow_alllibs

Instrument: Illumina HiSeq 2000

Strategy: WGS

Source: GENOMIC

Selection: unspecified

Layout: PAIRED

Spot descriptor:

1 forward101 reverse

Runs: 1 run, 797.3M spots, 114G bases, 60.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR2039641	797,326,308	114G	60.3Gb	2015-06-02

ID:: 1504902

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